NMR
Detailed NMR methods can be found in supplemental material. Briefly,
triple-resonance and NOESY spectra were collected on 0.1-1 mM protein
samples in PBS using either a 600 or 800 MHz Agilent DD2 console
equipped with cryogenically cooled probe. Backbone and side-chain
assignments and T1/T2 relaxation times for the oxidized samples were
obtained using standard triple resonance techniques employing either the
standard VARIAN Biopack or modified BEST-TROSY pulse sequences as
described previously[22-25].