Biophysical Characterization
Circular dichroism spectra were collected on 0.5 μM Fl J2LS, or 2 mM
A1LS, J2.1, J2.2, or J2.3 in PBS at 25oC using a Jasco
J-815 CD spectropolarimeter. Each spectrum represents the average of
four accumulations scanning at 20 nm/ min with a data-pitch of 0.2 nm.
Each spectrum was acquired from at least three trials representing two
biological replicates unless specified. Secondary structure prediction
was carried out using the BESTSEL web server [39, 40].
Samples of 46 uM or 23 uM FL J2LS in PBS were prepared and analyzed
using the SIBYL High-Throughput mail-in service. The resulting data was
analyzed using the ScÅtter software [40-45].