Figure
2. Compound 270 inhibits NF-κB transcriptional activity and directly
interacts with NF-κB in HEK 293 and RAW 264.7 cells.
(A) Chemical structure of 269 and 270. (B) The NF-κB transcriptional
inhibition activity of 269 and 270. (C) Effect of 270 on NF-κB protein
stabilization in HEK 293 cells. HEK 293 cells transfected with NF-κB
expression plasmid were treated with 5 μM 270 for 3 h before heated at
different temperatures. Protein levels were analyzed by western
blotting. (D) Protein band densities shown in (C) were quantified by
normalizing to β-actin. (E) Effect of 270 on NF-κB protein stabilization
in RAW 264.7 macrophages. RAW 264.7 macrophages were treated with 5 μM
270 for 3 h before heated at different temperatures. Protein levels were
analyzed by western blotting. (F) Protein band densities shown in (E)
were quantified by normalizing to β-actin. The data are presented as the
means ± SEM. All experiments were performed in three replicates.
Two-tailed Student’s t-test. *P < 0.05, **P <0.01,
***P < 0.001 versus DMSO.
Figure 3