Figure
3. Compound 270 suppresses the LPS-induced inflammatory response in RAW
264.7 macrophages.
(A) RAW 264.7 cells were pre-incubated with 270 for 4 h and then treated
with LPS (100 ng/ml) for 30 min. Total cell lysates were subjected to
western blot. (B) The protein levels shown in (A) were quantified by
densitometry analysis. (C) RAW 264.7 cells were treated with LPS (100
ng/ml) alone or together with 270 for 24 h. Relative mRNA level of each
pro-inflammatory gene was analyzed by use of RT-PCR and normalized by
GAPDH. The data are presented as the means ± SEM. All experiments were
performed in three replicates. Two-tailed Student’s t-test. *P
< 0.05, **P <0.01, ***P < 0.001 versus LPS
group.
Figure 4