Figure 6. Compound 270 improves the mice acute kidney injury
caused by intraperitoneal injection of LPS through attenuating renal
inflammation state
(A) HE staining of kidney sections after 24 h LPS-induced kidney injury.
Scale bar = 200 μm (n = 6). (B) The concentrations of plasma Crea and
BUN, (n = 6). (C) The relative mRNA level of NGAL and KIM1 in mice
kidney tissue and normalized by GAPDH, (n = 6). (D) The relative mRNA
level of each pro-inflammatory gene in renal tissues was detected by use
of RT-PCR and normalized by GAPDH, (n
= 6). (E) Western blot analysis of inflammatory signaling protein
expression in kidney tissues, (n = 6). (F) Relative protein expression
levels were determined by densitometric quantification of the
immunoblots shown in (E), (n = 6). The data are presented as the means ±
SEM. One-tailed Student’s t-test. *P < 0.05, **P
<0.01, ***P < 0.001 compared with the LPS group.
Figure 7