Experimental Approach:
LPS-induced macrophages were used to examine the anti-inflammation
activity of 270. Sepsis-induced AKI and ALI mice models were established
by intraperitoneal injection of LPS (10 mg/kg) for 24 h. Oral
administration 270 for 14 days before LPS stimulation. Plasma, kidney
and lung tissues were collected and used for histopathology, biochemical
assay, ELISA, RT-PCR, and western blot analyses.