Ultrasonic homogenizer extraction
Solvent (chloroform:methanol 2:1) was added to the dry algae sample and then homogenized in an ultrasonic homogenizer (Bandelin UW 3200/20 kHz) for 20 minutes (Byreddy et al., 2015). The lipid extraction was continued with Bligh and Dyer and Soxhlet methods.
At the end of each disruption method, the beakers in which lipid accumulates, were kept in the oven at 60 °C in order to remove the remaining solvent, then they were kept in a desiccator for 30 minutes and their final weighings were taken after cooling.
Crude lipid% = (Final Weight - Initial Weight) / Sample Weight x 100