Materials and methods
Reagents and
compounds
Compounds DNFB (MW: 516.45), 2-aminoethoxydiphenyl borate (2-APB),
capsaicin (Cap), ruthenium red (RR), GSK1016790A (GSK101), A-967079
(A-96) and allyl isothiocyanate (AITC) were purchased from Sigma-Aldrich
(St. Louis, MO). RR were made as stock solutions in before use internal
solution. Other compounds were made as stock solutions in DMSO before
use. Compounds used for the measurement of intracellular fluorescent
calcium were diluted in the Hanks’ balanced salt solution (HBSS).
Compounds were diluted in perfusion solution for patch-clamp recordings.
DNFB was dissolved in solvent (acetone/olive oil = 4:1) for
establishment of DNFB induced inflammation model (Bhol and Schechter,
2005).