Figure 2. Activation of TRPA1 channels by chemical DNFB in
calcium fluorescent assay. (a, b, c and d) Normalized increase of
intracellular Ca2+ levels in response to different
concentrations of DNFB (3-300 μM) or channel agonists of AITC (300 µM)
for TRPA1 (a), capsaicin (1 µM) for TRPV1 (b), 2-APB (200 µM) for TRPV3
(c) and GSK101 (0.1 µM) for TRPV4 (d) (n = 6).