2.9 Demographic history
We inferred demographic histories by applying the Pairwise sequentially Markovian coalescent (PSMC) method (Li & Durbin, 2011). Short-insert paired-end WGS reads of A. flavipes (two individuals), A. arktos , A. argentus , and M. melanurus were mapped to a repeat-masked S. harrisii genome. Putative X-linked scaffolds in the S. harrisii assembly were excluded (see Supplemental Methods). Maps were generated using the R package ‘ggmap’ (Kahle & Wickham, 2013).