Mice
Age- and sex-matched C57BL/6 mice, C57BL/6-Foxp3GFP reporter mice, RORγtfl/fl mice, and Foxp3creRORγtfl/fl mice (age: 6-12 weeks) were used in our experiments. RORγtfl/fl mice were purchased from The Jackson Laboratory (USA). C57BL/6-Foxp3GFP reporter mice and Foxp3cre mice were provided by B. Malissen (Université de la Méditerranée, Marseille, France) and A. Yoshimura (Keio University, Japan), respectively. All mice were maintained under specific pathogen-free conditions. All experiments described in this report were performed according to the Guide for the Care and Use of Laboratory Animals at the University of Tsukuba and were approved by the Animal Ethics Review Committee of the University of Tsukuba.