Figure 2. Infiltration of Tr17 cells into ankle joints after
onset of CIA through high expression of CCR6
a) Lymphocytes were harvested from draining lymph nodes on 10, 42, and
56 days and from ankle joints on 42 and 56 days after immunization of
CII emulsified with complete Freund’s adjuvant in C57BL/6 mice. a) The
expression of RORγt in Foxp3+Treg cells was
chronologically analyzed using flow cytometry. b) Bar graph shows
percentage of Tr17 cells in LN and AJ on day 10, 42, and 56. c) At 10
days after CII immunization CCR6 expression on Tr17 cells,
RORγt-Treg cells, and RORγt+non-Treg
cells in lymph node were analyzed using flow cytometry.