Production of MSTN mutated cattle using CRISPR–Cas9
Gyeong-Min Gim1,2, Dong-Hyuk
Kwon1,2, Kyeong-Hyeon Eom1,2, JoonHo
Moon3, Ji-Hyun Park3, Won-Wu
Lee3, Dae-Jin Jung4, Dae-Hyun
Kim4, Jun-Koo Yi4, Jae-Jung
Ha4, Ka-Yeong Lim5, Jin-Soo
Kim5, Goo Jang1,2
1Laboratory of Theriogenology and Biotechnology,
Department of Veterinary Clinical Science, College of Veterinary
Medicine and the Research Institute of Veterinary Science, Seoul
National Univeristy,
2BK21 Plus program, College of Veterinary Medicine,
Seoul National University, Seoul, Republic of Korea
3LARTBio Inc., Seoul, Republic of Korea
4Gyeongsangbukdo Livestock Research Institute,
Yeongju, GyeongSang Buk-Do 36052, Republic of Korea
5Center for genome engineering, Institute for Basic
Science (IBS), Seoul 08826, Republic of Korea
Correspondence: Goo Jang, 1 Gwanak-ro, Gwanak-gu, Seoul,
Republic of Korea, 08826
E-mail:snujang@snu.ac.kr
Tel: 82-2-880-1280, Fax: 82-2-873-1269
Keywords: Bovine embryos, CRISPR-Cas9, In vitro fertilization,MSTN , Microinjection
Abbreviations: MSTN, myostatin; sgRNA, single guide
RNA; ART, advanced reproductive technologies; SCNT,somatic cell nuclear transfer; T7E1, t7 endonuclease 1