Enzymatic treatment of biofilm
Biofilms were grown in a 96-well plate as described above. Wells were
either treated with 2 U/ml of DNase, 15 µg/ml proteinase K or
3 µg/ml of glucosidase (Sigma
G4511-100UN) to target eDNA, protein or polysaccharide components of
each biofilm. Treatments occurred at the time of inoculation. Biofilm
was determined using CV as previously described.