Enzymatic treatment of biofilm
Biofilms were grown in a 96-well plate as described above. Wells were either treated with 2 U/ml of DNase, 15 µg/ml proteinase K or 3 µg/ml of glucosidase (Sigma G4511-100UN) to target eDNA, protein or polysaccharide components of each biofilm. Treatments occurred at the time of inoculation. Biofilm was determined using CV as previously described.