2.1 | Samples and DNA sequencing
We analyzed resequenced whole genome data from 90 S. catenatusindividuals from nine of the populations described in Ochoa et al.
(2020) (see Table S1 for population names, codes, and numbers of
individuals analyzed, and Figure 1 in Ochoa et al. [2020] for map
showing population locations). We also analyzed samples from a
population of S. tergeminus from Cheyenne Bottoms, Kansas (Table
S1) as a reference for expected patterns of variation in a
closely-related yet outbred species (see above).
We used genomic DNA previously extracted from blood samples (see Sovic
et al. [2019]) to prepare paired-end (PE) shotgun libraries with
500-bp inserts and to generate PE 150-bp reads using Illumina sequencing
platforms. Most samples were sequenced at medium or low coverage
(< 15x), but a small number of samples were sequenced at high
coverage (> 15x) (see Table S1 for mean coverage statistics
for individual samples).