Sandfly iDNA metabarcoding
We captured 56,775 sand flies and sorted them into 1,137 pools of 50
flies. The number of sandflies captured at each site ranged
substantially from 131 individuals at site E19 to 8,469 individuals at
site B5 (site level data summary available in Appendix S1: Table S1).
Metabarcoding results filtered for sandfly species (Family: Psychodidae)
revealed 34,598,149 total paired sequence reads from 927 pooled sandfly
samples (the number of molecularly processed samples is less than the
total number of samples due to the exclusion of some pooled samples from
sites with disproportionately high number of sand flies (see Appendix
SI: Table S1 for details). After quality control measures designed to
remove non-amplified samples and clean the raw sequencing data, the
final dataset used for analysis had 19,334,956 total paired sequence
reads from 851 pooled samples from across all 39 sites. Metabarcoding
results for vertebrate species revealed 25,093,673 total paired sequence
reads from 976 pooled sandfly samples. After quality control measures
designed to remove non-amplified samples and clean the raw sequencing
data, the final dataset used for analysis had 4,753,773 total paired
sequence reads from 481pooled samples which represented 38 of the 39
sites.