Cytokine cytometric bead array (CBA)
Serum was obtained by centrifugation of blood samples at 3000 rpm for 15 min at 4 °C. The levels of IL-1β, IL-4, IL-6, IL-10, IL-12p70, L-17A, and tumor necrosis factor (TNF)-a were measured using CBA kits (BD Bioscience, USA) as the manufacturer’s instructions.