Animals
Male C57BL/6J mice, 5-6 weeks old, were purchased from the Beijing Vital
River Laboratory Animal Technology Co., Ltd. (Beijing, China). Mice were
housed in pathogen-free conditions and fed with food and water ad
libitum. The study was approved by the local ethics committee of The
First Hospital of Jilin University, Changchun of China and all
experimental procedures complied with the regulations for the management
of laboratory animals in the Jilin Province (Ethical approval number:
2017-216).
Reagents
The neuritogenic P0 peptide 180-199 was synthesized by
GenScript (USA). BAY11-7082 was
purchased from Beyotime (China).
Antibodies against NF-κB p65 and NF-κB phosphorylated p65 were purchased
from Cell Signaling Technology (USA).