BAY11-7082 treats EAN
BAY11-7082 (Beyotime, China) was dissolved in 1% dimethyl sulfoxide (DMSO, USA) in PBS. EAN mice were divided into three groups. For preventative treatment, BAY11-7082 solution was administered by intraperitoneal injection (20 mg/kg) from day 1 to days 16 post-immunization (p.i.) by every two days. For therapeutic treatment, BAY11-7082 solution was administered by the same way as the preventative treatment at the same dose from the day on which the onset of symptoms was observed to day 30 by every two days. Control animals with EAN received the same volume of the vehicle solution (1% DMSO in PBS). Using the same protocol descripted as the above assessed the clinical signs of mice.