Supplemental data:
The following materials are available as supplemental data.
Supplemental datasets S1 and S2 : Relative abundance of all
identified metabolites in C. reinhardtii and UWO241 grown at
different steady-state temperatures and heat stress.
Supplemental dataset S3: A summary of the differentially
expressed genes (DEGs) in steady-state grown UWO241.
Supplemental dataset S4 : Summary of the differentially
expressed genes (DEGs) in UWO241 grown at grown at 4°C, 10°C and 15°C
and exposed heat stress (24°C) for 6 hours.
Supplemental dataset S5 : KEGG analysis of the pathways
significantly up- or down-regulated pathways in heat-shocked UWO241.
Supplemental dataset S6 : The Heat Shock Protein (HSP) gene
family in chlorophyte, including a summary of the characteristics of the
predicted HSPs in UWO241 and their expression profiles under steady
state and heat stress.
Supplemental Figure S1. Light microscopy images ofChlamydomonas cells stained with 0.5% Evans Blue.
Supplementary Figure S2. Chlorophyll loss in UWO241 andC. reinhardtii acclimated to different growth temperatures and
exposed to non-permissive conditions (24°C and 42°C).
Supplementary Figure S3. Light microscope images of C.reinhardtii acclimated to different steady state temperatures
(10°C, 28°C, 37°C) and exposed to non-permissive temperature (42°C)
Supplementary Figure S4. Cladograms representing phylogenetic
relationships of the HSP genes in UWO241.
Supplementary Figure S5: A full protein alignment of the key
domains in the cytosolic HSP70A proteins present in the UWO241 genome.
Supplementary Figure S6: Representative western blots showing
the accumulation of HSPs in cultures of UWO241 and C. reinhardtiiat different steady-state temperatures.