<div>This study aims to quantify the utility of allozyme supernatant and
formalin-fixed tissue samples for SNP-based analyses using ten specimens
of spring salamanders (<i>Gyrinophilus porphyriticus</i> ). We use a
target capture approach (RAD-based capture) with replicated sampling
(allozyme supernatant, allozyme homogenate pellet, and formalin-fixed
tissue) as well as standard RADseq-derived loci from frozen blood of
seven of the same ten specimens. In particular we seek to investigate:
(1) what factors predict success of allozyme supernatant and
formalin-fixed samples to genomic inquiry? (2) How similar are SNP-calls
for differently preserved technical replicates? (3) Do replicates for a
given specimen yield consistent results across phylogenomic and
population genomic analyses? 4) What potential biases are introduced by
pooling target-capture and RADseq datasets?</div>