Fig. 2. The fabricated device showing a fish loaded in trap A and simulation overlays of the electric voltage, pressure drop and shear stress for traps B, C, and D respectively. The wire for electric stimulation running through the indirect flow channel is highlighted in red
In all experiments with zebrafish larvae presented in the following sections, the control groups with no off-chip chemical exposure and no on-chip electric stimulation demonstrated no movement in the device with zero RD and zero TBF. The responses observed in the test groups was therefore associated with the effect of electric stimulation and the assayed chemicals in Table 1 (Supplementary video2).