3.1. Performance of the quadruple zebrafish microfluidic device
Compared to our single-fish device reported
previously[16], the design modifications to the
quadrupole-fish device in this paper had an impact on the electric
signal distribution and the fluid flow through the device, meriting
characterization.
The indirect flow offered a convenient way of steering the fish from the
main channel to fill the open TRs. An example of how each fish was
trapped in place is seen in trap A in Fig. 2. The quadruple-fish device
functionality and success rate were assessed based on the testing time
per fish, loading and orientation efficiencies defined by Eq. (3), (4)
and (5), respectively.
\(Testing\ time\ per\ fish=\frac{Loading\ time+60\ s\ recovery\ period+20\ s\ stimulation\ interval}{Number\ of\ fish\ oriented\ tail-first\ in\ the\ device}\ \)