Figure captions
Fig. 1. Sampling plots distributed within the study site -i.e.
CK: control; r8: 8 years since reclamation; r11: 11 years since
reclamation; r14: 14 years since reclamation; and r17: 17 years since
reclamation. Figs. A, B and show the study site in Zoucheng, China. The
D pictures is from the subsidence area in eastern China.
Fig. 2. Soil key attributes in the reclaimed (i.e. r8, r11, r14
and r17) and control (iCK) plots. Mean ± standard deviation (SD) (n =
15). Letters indicate significant differences among the 5 sites. A: SOM;
B: soil pH value; C: soil EC value; D: soil AK content; E: soil OP
content; F: soil TN content; G: soil URA activity; H: soil PPO activity;
I: soil DHG activity; J: soil FDA activity. The values in the picture
represent the significance of T-test; when the number < 0.05,
it means significant; the value < 0.01: very significant; the
value < 0.001: extremely significant.
Fig. 3. Taxonomic distributions of the most abundant (A) phylum
and (B) genera levels of the SMCs found in the studied sites after
reclamation (i.e. r8, r11, r14, r17) and control sites (CK).
Fig. 4. Molecular ecological networks (MENs) retrieved for the
sampling plots subjected to land reclamation 8 (r8; A), 11 (r11; B), 14
(r14; C), and 17 (r17; D) years before sampling and control plot (CK;
E). The nodes are plotted in circles of different sizes. The established
nodes are portrayed in colors (i.e. purple, yellow, blue, green,
….). Each node corresponds to one microbial population of the
most abundant phylum. The identified modules are labeled with numbers. A
pink connector indicates a negative correlation, while a light-blue
connector indicates a positive correlation between modules. Please, see
online version for colors. CK: control; r8: eighth years after
reclamation; r11: eleventh years after reclamation; r14: fourteenth
years after reclamation; r17: seventeenth years after reclamation
Fig. 5. Z-P plot showing the keystone species (i.e. module hubs
and connectors) in the different molecular ecological networks (MENs).
Different symbols with special colors
represent different networks as follows: green square for the CK
network, red star for the r8 network, blue upward-facing triangles for
the r11 network, magenta downward-facing triangles for the r14 network
and cross cirle for the r17 network. The module hubs and connectors are
labeled with phylogenetic affiliations (Acido-Acidobacteria,
Actino- Actinobacteria, Bacteroi-Bacteroidetes, Chloro-Chloroflexi,
Depen-Dependentiae, Elusi-Elusimicrobia Gemma-Gemmatimonadetes,
Nitro-Nitrospirae, Patesci-Patescibacteria, Plancto-Planctomycetes,
Proteo-Proteobacteria and Verru-Verrucomicrobia ). CK: control; r8:
eighth years after reclamation; r11: eleventh years after reclamation;
r14: fourteenth years after reclamation; r17: seventeenth years after
reclamation.
Fig. 6. Correlation between the identified molecular ecological
networks (MEN) modules and the key soil attributes for the five study
plots: (A) r8; (B) r11; (C) r14 and (D) r17; (E) CK. CK: control; r8:
eighth years after reclamation; r11: eleventh years after reclamation;
r14: fourteenth years after reclamation; r17: seventeenth years after
reclamation. * mean significant difference at 5% (p<0.05), **
mean significant difference at 1% (p<0.01), *** mean
extremely significant difference at 1% (p<0.001).