Patients selection and data collection
Patients affected by NB treated from March 2005 to March 2019 at the Bambino Gesù Children Hospital of Rome, Italy, were retrospectively enrolled. The patient’s disease was staged according to age at the time of diagnosis (< or ≥ 18 months), INSS [2] and the INRGSS [3]. The amplification of MYCN gene was determined by fluorescent in situ hybridization (FISH) [25]. Clinical and pathological data of all cases were retrieved from the medical records available in the institutional archives. The inclusion criteria were defined as follows: (i) patients affected by NB; (ii) patients who underwent MYCN gene determination; (iii) patients with at least 6 months of follow up (iv) patients images had to be stored in Dicom format.