Western blot analysis and Co-immunoprecipitation (co-IP) assay
Western blot and Co-IP were performed as described previously (L. Wang et al., 2018). The primary antibodies for IRE1α (ab37073, 1:1000), p-IRE1α (ab48187, 1:1000), PERK (ab79483, 1:1000), eIf2α (ab264254, 1:1000), ATF4 (ab23760, 1:1000), p38 (ab170099, 1:1000), p-p38 (ab178867, 1:1000) and p-Akt (ab38449, 1:800) were purchased from Abcam. Antibodies against ATF6α (p50) (#65880, 1:1000), p-PERK (#3179, 1:1000) and p-eIf2α (#3398, 1:1000) were purchased from Cell Signaling. Antibodies about CREB3L2 (14514-1-AP, 1:800), Akt (10176-2-AP, 1:2000), GRP78 (11587-1-AP, 1:2000), GFP tag (50430-2-AP, 1:1500), CHOP(15204-1-AP, 1:500), SREBP1 (14088-1-AP, 1:1000) were purchased from Proteintech. β-Actin (AF5003, 1:1000) was purchased from Beyotime. was purchased from Santa Cruz. Samples were subjected to standard SDS-PAGE, and the resulting bands were transferred onto polyvinylidene difluoride membrane for visualizing specific proteins. The ECL Plus detection system (Thermo Scientific, USA) was used for detection.