Pharmacokinetics and pharmacogenetics of F-araA
Heparinized peripheral blood (5mL) was collected from the patients
before and after the start of F-araA infusion on day -5 at specific time
points (n=5). The plasma was obtained by centrifuging at 3000rpm for 5
minutes and stored at -80oC until analysis. F-araA
levels in plasma samples were measured by LC-MS/MS as reported
previously 9. Similar to our previous study9, selected polymorphisms in the NT5E(rs2295890) and deoxycytidine kinase, DCK (rs11544786) genes (with an
allele frequency of >0.1 based on 1000 genome database or
with clinical significance) encoding the rate-limiting enzymes in the
F-araA metabolic pathway were screened using the pre-HCT genomic DNA by
followed by Sanger sequencing.