Figure Legends

Figure 1: Growth and metabolic characteristics of WT and BCAT1 KO clones in fed-batch cultures. A.) Viable Cell Density, B.) Viability, C.) Lactate, D.) Glucose, E.) Titer, F.) Specific Productivity, G.) isovalerate H.) isobutyrate, I.) 2-methylbutyrate. BCAT1 KO Clone 83 (), BCAT KO Clone 90 ( ), WT Clone 47 ( ). For WT Clone 47, solid and dotted lines represent duplicates.
Figure 2: Growth and metabolic characteristics of WT and BCAT1 KO clones in HiPDOG cultures. A.) Viable Cell Density, B.) Viability, C.) Lactate, D.) Glucose, E.) Titer, F.) Specific Productivity, G.) isovalerate H.) isobutyrate, I.) 2-methylbutyrate. BCAT1 KO Clone 83 (), BCAT KO Clone 90 ( ), WT Clone 47 ( ). For WT Clone 47, solid and dotted lines represent duplicates.
Figure 3: Levels of other metabolic byproducts across fed-batch and HiPDOG cultures of BCAT1 KO clones. A.) 3-phenyllactate, B.) 4-hydroxyphenyllactate, C.) indole-3-lactate, D.) butyrate, E.) formate and F.) glycerol concentrations in fed-batch and HiPDOG cultures of BCAT1 KO Clone 83 () and BCAT1 KO Clone 90 ( ). Solid and dotted lines represent fed-batch and HiPDOG cultures, respectively.
Figure 4: Effect of lactate on growth of CHO shake-flask batch cultures. A.) BCAT1 KO Clone 83 and B.) WT Clone 47 viable cell density over 6 days at added lactate concentrations ranging from 0 to 10 g/L. Lactate concentrations gradually increased by roughly 2 g/L during the culture for control, osmolality control and 2.5 g/L lactate conditions but did not change substantially for higher concentrations (data not shown). All conditions were run in triplicate. Error bars represent one standard deviation.