2.8 Acetylation of fatty alcohols
The purified fatty alcohol mixture was acetylated by using a 1.2 molar
equivalent of acetic anhydride and 0.1 equivalent of sodium acetate
anhydrous (equivalents were based on 14:OH). The reaction mixture was
allowed to stir for 1 h at 80°C in a water bath and then transferred to
a separatory funnel where it was diluted with ethyl acetate and washed
with a saturated solution of sodium bicarbonate. The organic phase was
dried with anhydrous sodium sulfate, and the solvent evaporated in
rotary evaporator.