Figure
3 . GMP-compliant reagents improve T cell phenotype and
function . Samples taken at day 30 harvest from G-Rex10 flasks
(stimulation round 3 + 9 days) analysed as before. (A ) Direct
comparison of representative line LAD1 cultured using GMP-compliant
reagents and standard culture reagents demonstrated both T cell cultures
had comparable expression of most markers, but reduced CD57 expression
in GMP-compliant process. (B ) Analysis of mean surface marker
expression showed a significantly higher percentage of CD62L+/CD45RO+
Central Memory T cells in GMP-compliant culture (n=4) compared to
standard (n=4, p=0.002). Intracellular staining showed no significant
difference in (C ) cytokine expression between the two groups,
or (D ) relative frequency of differentiated T cell
subpopulations by cytokine expression. Data are represented as means ±
SEM.