2.4 Statistics
Statistical analyses were performed using Microsoft Excel 2007, SPSS Statistics 23.0 (IBM, Armonk, New York, USA) and R package vegan (version 2.5-5). Adobe Illustrator CC 2020 and R package ggplot2 (version 3.2.0) were used to draw figures.
The average value of all parameters was taken from three replicates, and the standard error was calculated. One-way analysis of variance (ANOVA) was used to analyze the data (p≤0.05). Correlation analysis used the Mantel test and Spearman’s rank correlation coefficients. LDA Effect Size analysis (LEfSe) used the ANOVA and then the Wilcoxon rank sum test to analyze the differences among the treatments (threshold set to 0.05).