2.2.3 Open Field (OF)
Emotional alterations and locomotor activity were evaluated by the OF test using a white plywood apparatus (50 × 50 × 25 cm) as previously described (Archer, 1973; Griñan-Ferré et al., 2016).The apparatus’ ground was divided into the center and peripheral area. Each individual was placed at the centre of the open field and allowed to explore the apparatus for 5 min. The apparatus was cleaned with 70% ethanol after between trials. The behaviour was recorded and later analysed with SMART ver. 3.0 software (Panlab). The locomotor activity of the mice calculated as the sum of total distance travelled in 5 min, the centre stay duration and the number of rearings were evaluated.