Statistical analysis
Data are presented as the mean ± standard error of the mean (SEM).
Significant differences in the abundance of proteins in different
tissues were identified using one-way ANOVA. Significant differences
were determined when P <0.05. The intensity of
Nav1.5 staining was analyzed in different areas of the
AV junction, including RN, CN, INE, atrial and ventricular tissues,
using quantitative immunofluorescence staining analysis. The background
level of staining in each tissue section can affect the results obtained
from immunofluorescence staining; thus, the staining intensity of
Nav1.5 in
ventricular
tissue was included and used as a reference in each section used for
analysis.