Skin tests
All children underwent skin prick testing (SPT) to mosquito with commercial extract of Aedes communis (Stallergenes, Anthony, France), along with common aeroallergens for our region (9), including house-dust mites (Dermatophagoides pteronyssinus andDermatophagoides farinae ), grass pollen mix (Phleum pratense, Poa pratensis, Dactylis glomerata, Lolium perenne, Festuca pratensis, and Avena eliator) weed pollen mix (Artemisia, Urtica, Taraxacum, Plantago) tree pollen mix (Alnus glutinosa, Corylus avellane, Populus alba, Ulmus minor, Betula alba) molds (Alternaria, Cladosporium, Penicillium, and Aspergillus) and animal dander (cat and dog). Histamine (10 mg/ml of histamine phosphate) and 0.9% saline were used as positive and negative controls, respectively. Weal 3 mm greater than negative control was considered a positive reaction.