Feeding
In the 14-day acclimation, fish at 21g/kg appeared to increase feeding
compared to the control, although this trend was not significant by the
conclusion of the experiment (Figure 1E). The 55g/kg treatment feeding
behavior was very similar to the control. Both the 85g/kg and 105g/kg
treatments decreased towards complete cessation of feeding as the target
salinity was reached. For extended constant salinity at 85g/kg, feeding
dropped to near zero over the 14-day acclimation period before rising
slightly and finally falling back towards zero as fish reached MP. At
105g/kg constant salinity feeding dropped to near zero during
acclimation and remained near zero until MP. At 75g/kg, feeding dropped
to near zero during the acclimation period but recovered to near the
feeding rate of the handling control, although at 12-weeks feeding was
still significantly lower (p = 0.0093) than the control (Figure 2C).