Parallel quantitation of mRNAs by transcriptomics
In total, 35902 unique transcripts were quantified. The average read length was 80 bp, with a minimum of 5.5 million reads per lane with 4 lanes for each sample. The FASTQ analysis was successful after trimming and adaptors removal for all the sequenced samples.
Analysis for DEG resulted with 17 salinity-dependent transcripts (p-adj < 0.05). From them, 8 transcripts were up-regulated and 9 were down-regulated (Table 2). Within the up-regulated genes, enrichments for membrane components such as transporters, nucleic acid binding, dehydrogenases/reductases and one immunoglobulin domain-containing protein were present. The down-regulated genes included enrichments notably for regulators of transcription by binding RNA or DNA, signal transduction, endopeptidases for proteolysis and integral components of membrane such as microtubules.