Genesis clustering
Protein regulation patterns were clustered in a non-biased manner using
Genesis 1.8.1 (Sturn, Quackenbush, & Trajanoski, 2002). Proteins with
similar regulation patterns in individual replicates were clustered to
identify non-significant proteins which nonetheless followed the same
regulation patterns as significant proteins. Six clusters were
determined to be the ideal number which grouped the majority of all
significantly regulated proteins in two groups, one containing most
up-regulated proteins and the other containing most down-regulated, as
well as isolating the majority of proteins in clusters with regulation
patterns that did not follow treatment groupings.