Comparison of protein and transcript regulation
Mean protein abundances per treatment group were compared for each unique accession number with the corresponding mean mRNA abundance. Fold change (FC), the abundance difference between treatments expressed in multiples of the control, was transformed by the log2 and plotted with mRNA abundance along the X-axis as the independent variable and protein abundance along the Y-axis as the dependent variable based on mRNA-protein correlation analysis convention (Buccitelli & Selbach, 2020). Additionally, significantly regulated (adj. p<0.05) proteins were compared as a subset in the same manner, with the degree of correlation in each case being the coefficient of determination (R2).