Figure caption
Fig. 1. Feedback feeding platform in SLs fermentation process.
The solid lines represent material pipelines, dotted lines represent
signal pipelines.
Fig. 2. Process parameters of SLs fermentation with different
oil feeding modes. A: SLs titer; B: phased SLs productivity; C:
difference in total SLs production; D: process CER and OUR profilings
Fig. 3. Metabolic characteristics of SLs at different
production stages. A: The effect of the product on the synthesis of SLs;
B: Changes in RQ during SLs synthesis; C: Relation between SLs
concentration and viscosity.
Fig. 4. Analysis of SLs synthesis stage.
Fig. 5. Intelligent feedback feeding model. OUR for the oxygen
uptake rate (mmol/L/h), CER for the CO2 release rate
(mmol/L/h), RQ for the respiratory quotient, RPM for the fermentation
speed (rpm), DO for the dissolved oxygen (%), Temp for the fermentation
temperature (℃), pH for the fermentation pH. SLs represents the
productivity of SLs (g/L/h), Oil represents the consumption rate of
rapeseed Oil (g/L/h), Glc represents the consumption rate of glucose
(g/L/h), V represents the working volume (L), c1 and c3 represent the
concentrations of rapeseed Oil and glucose in the actual fermentation
broth (g/L), c2 and c4 represent the controlling concentrations of
rapeseed oil and glucose in fermentation broth (g/L).
Fig. 6. Feedback feeding control. A: Glucose concentration; B:
Rapeseed oil concentration.
Fig. 7. Semi-continuous fermentation of SLs production with the
assistance of intelligent feedback feed model. A: Rapeseed oil and
glucose concentration as well as DCW; B: SLs titer; C: SLs productivity
and yield.