Parasites, while being among the most diverse modes of life, are often disregarded in conservation strategies39. It is well established that parasites affect the stability of food webs and ecosystem health, but hyperparasites have thus far been severely understudied. For future studies on host associations across multiple trophic levels and on the effects of climatic conditions and land-use changes, parasites and hyperparasites are part of our DarkCideS 1.0 database. The fourth dataset lists the parasitic bat flies and their Laboulbeniales fungal hyperparasites associated with cave bats. Data were collected from several sources, including our own fieldwork36, Haelewaters et al.40, and de Groot et al.41. Bat fly taxonomy followed Dick and Graciolli42 and Graciolli and Dick43 and fungal taxonomy followed Index Fungorum44. In addition to the conspicuous bat flies, bats are host to several other lineages of parasites mites and ticks, lice, fleas, bugs, and earwigs45,46. Consequently, the fourth dataset will be expanded on in future versions of DarkCideS with data on these parasitic organisms. A recent call for global collaborations among bat scientists and collaborations to generate multitrophic data of bats, bat flies, and fungi45 along with the current DarkCideS 1.0 initiative will contribute to a general understanding of how ecological and life-history traits are correlated with bat parasitism and how host associations may change under changing conditions.