Figure 7. Proteins identified in each band of SDS-PAGE gel resolved proteins by LC-MS/MS.
Figure 8. EpiMatrix ClustiMer immunogenicity scale. Clustimers derived from residual proteins in API vary in their calculated immunogenicity risk. Clustimers with low human homology and their scores were identified and calculated in the EpiMatrix program. Clustimers were binned according to their score and are displayed on the right side of the scale. Number in parenthesis indicates multiple peptides from a given protein are included in the range. Foreign proteins and human CLIP are shown on the left side of the scale to give relative risk. Immunogenicity risk increases from green (minimal) to red (high). Abbreviations: HCV= Hepatitis C virus, CLIP= Class II-associated invariant chain peptide. EBV=Epstein-Barr virus. TRPCIF=Transcriptional Regulatory Protein CpxR IcIR Family, AHRC= Alkyl Hydroperoxide Reductase C, 30SRPS10= 30S Ribosomal Protein S10, CspC= Cold Shock-like Protein C.
Figure 9. MK-1454 API isolated and purified with the new process resolved on SDS-PAGE with silver stain (a), Coomassie blue stain (b).BSA, bovine serum albumin, MK-1454 157, 165, 166 are three small scale biocatalytic API isolated with the new workup procedure. C-10 mg/mL, represents a chemically synthesized MK-1454 control that is free of protein impurities.