Annexin V/Propidium Iodide Cell Viability Assay
Samples of approximately 100,000 cells were spun down at 400 g for 4 minutes, then aspirated and resuspended in BioLegend Cell Staining Buffer two times before finally pelleting and resuspending the cells in 20 µL of BioLegend Annexin V Binding Buffer. 1 µL of propidium iodide (Ex/Em = 493/636 nm) and 0.5 µL of annexin V R-PE (Ex/Em = 565/578 nm) (Biotium #29045) were added to each cell sample and incubated in the dark for 20 minutes. Binding buffer (80 µL) was then added to each sample and then samples were transferred to a 96-well plate for flow cytometry.