Conclusions
Overall, this study shows that Lipofectamine can be used to transfect a
moderately high percentage of Jurkat cells (51±9.7%) but a much lower
fraction (8.1±0.8%) of primary T cells. Nonetheless, this modest
transfection efficiency may still be suitable for the generation of
CAR-T cells, since some studies using lentiviral vectors have obtained
complete responses with transduction efficiencies as low as 2.3%
CAR+ T cells.81 In either case, our
results emphasize that cellular uptake (due to a lack of HSPGs) and an
increased risk of apoptosis induced by the PYHIN DNA sensors AIM2 and
IFI16 are two particularly important issues that should be considered in
the development of both viral and non-viral gene delivery methods for T
cells.