Statistical analysis
Frequencies of lymphocyte subsets and usage of each TCR-Vβ family
between patients with AIN and age-matched control subjects were compared
using the Wilcoxon rank-sum test.
In the analysis of the skew of usage of TCR-Vβ families, we used the
χ2 test and Wilcoxon rank-sum test. The Wilcoxon
signed-rank test was used to analyze the different usages of TCR-Vβ
families between conventional T cells and Tregs. Two-sided p-values
< 0.05 denoted statistically significant differences.
Statistical analysis was performed using the JMP12 (SAS Institute Inc.,
Cary, NC, USA) software.