Change of preferent usage of TCR-Vβ families between
conventional T cells and Tregs
Recently, it has been reported that the TCR repertoire in Tregs is
highly as diverse as one in conventional T cells and the usages of
TCR-Vβ families overlapped between these T cell subsets. Therefore, the
usage of 24 TCR-Vβ families was individually assessed between
conventional T cells and Tregs in both control subjects and patients
with AIN (Figure 5 ). Increased usages of TCR-Vβ 3, 7.1, 8, 9,
13.1, 16, 17, 20, and 22 were observed in conventional T cells compared
with Tregs in both control subjects and patients with AIN. In contrast,
the usages of TCR-Vβ 5.1, 5.2, 5.3, 12, 13.2, and 13.6 in Tregs were
significantly increased compared with those observed in conventional T
cells. These results were summarized in Figure 6 . Although
there was a certain preference of usages of the TCR-Vβ repertoire
between conventional T cells and Tregs in both control subjects and
patients with AIN, usage of the TCR-Vβ 9 family was prominently
different between control subjects and patients with AIN. All control
subjects showed that the usage of TCR-Vβ 9 in conventional T cells was
higher than that observed in Tregs. In contrast, patients with AIN did
not demonstrate consistent variation between conventional T cells and
Tregs. The highly increased usage of TCR-Vβ 9 in Tregs was observed in
four patients with AIN in whom neutropenia persisted (Patient 2,3,4,7).
There were no significantly different usages noted in the remaining 23
TCR-Vβ families between control subjects and patients with AIN.