Dynamic light scattering
DLS was used to detect aggregates in the size range from about 1 nm to 1
µm. DLS was performed on a Malvern Zetasizer Nano (Malvern, Herrenberg,
Germany). 500 µL of each sample in a concentration of 10 mg/mL was
analyzed in plastic cuvettes at 25◦C using the automatic mode (n=3).
Z-average diameter and polydispersity index were calculated using
Dispersion Technology Software version 7.03 (Malvern, Herrenberg,
Germany).