Time point 0h 0h 0h 4h 4h 4h
Condition
Vial
Syr.
Syr. + MN
Vial
Syr.
Syr. + MN
UV spectroscopy a/b ratio 1.46 1.46 1.45 1.38 1.41 1.44
DLS Z-average diameter 3.76 3.68 3.99 3.59 3.61 4.23
in nm (SD) (0.01) (0.03) (0.33) (0.03) (0.07) (0.04)
Polydispersity index 0.191 0.203 0.191 0.188 0.177 0.182
(SD) (0.003) (0.039) (0.002) (0.001) (0.080) (0.009)
HP-SEC
Monomer content (%)
99.8
98.0
98.0
99.6
99.6
99.6
Dimer content (%)
0.2
2.0
2.0
0.4
0.4
0.4
Molecular weight monomer (kDa)
157
150
153
155
155
155
NTA size estimation
Mean in nm (SD) 429 (233) 408 (180) 463 (330) 386 (182) 391 (234) 352 (179)
MFI
Particles ≥ 2 µm per mL
3064
2948
2948
2434
2376
1773