Figure 1: Overview of method . A novel combination of a metabolic and statistical models forecast the time-course amino acid consumption profiles in CHO cell cultures, as follows: (1) Routine bioprocess measurements are used as input data. (2) A metabolic model initially estimates early amino acid consumption rates.(3) A regression model refines these predictions. (4) These refined predictions are fit to a curve describing the time-course profile. (5) This would allow for anticipatory control of amino acid availability in bioreactors.