Figure 3. LCZ696 treatment and TLR2 knockdown attenuated doxorubicin-induced cardiac inflammation in mice.
A. Representative images of anti-TNF-α staining in the hearts of each group (200×). B. TNF-α levels in mouse heart tissue homogenates determined by ELISA. C . Western blot analysis of IκBα levels in heart tissue. GAPDH was used as a loading control.D. Nuclei were isolated from mouse heart tissue, and NF-κB in the nucleus was detected by western blot. Lamin B and GAPDH were used as controls. E-G. Real-time PCR was used to determine the mRNA levels of Tnfa (E), Mcp1 (F), and Il-6 (G) in mouse heart tissue (data were normalized to Actb ). (n=7; ***P <0.001 vs WT control group; ###P <0.001 vs DOX group).