Figure 6. LCZ696 treatment attenuated doxorubicin-induced
cardiac injury by inhibiting TLR2-MyD88 complex formation.
A. Time course of the DOX-induced TLR2-MyD88 interaction in
H9C2 cells. Cells were treated with 1 µM DOX for up to 1 h.
Quantification is shown below. B. H9C2 cells were pretreated
with LCZ696 (20 μmol/l) for 1 h and then stimulated with DOX (1 μM) for
30 min. The TLR2-MyD88 interaction was analyzed. Quantification is shown
below. C. H9C2 cells were transfected with control siRNA (NC)
or TLR4 siRNA (siTLR4) and then treated with DOX (1 μM) for 15 min.
TLR2-MyD88 interactions were analyzed. Quantification is shown below.D. H9C2 cells were transfected with control siRNA (NC) or MD2
siRNA (siMD2) and then treated with DOX (1 μM) for 15 min. TLR2-MyD88
interactions were analyzed. Quantification is shown below. (n=5; ***P <0.001 vs Ctrl group; ### P <0.001
vs DOX group).