Figure 2. LCZ696 treatment and TLR2 deficiency attenuated doxorubicin-induced cardiac fibrosis.
A. Representative images of Sirius Red and Masson trichrome staining of longitudinal sections of the hearts (200×). B-C.Quantification of interstitial fibrotic areas (%) from Sirius Red-stained heart sections (B) and Masson’s Trichome staining (C).D-E. The mRNA levels of Col1a1 (D) and Tgfb (E) were detected by real-time PCR in the heart tissue from the above transplanted mice (data were normalized to Actb ). F.Heart tissue from each group of mice was homogenized for western blot analysis of collagen I, TGF-β and GAPDH. (n=7; ***P <0.001 as indicated, and ns is not significant).