Figure 3. LCZ696 treatment and TLR2 knockdown attenuated
doxorubicin-induced cardiac inflammation in mice.
A. Representative images of anti-TNF-α staining in the hearts
of each group (200×). B. TNF-α levels in mouse heart tissue
homogenates determined by ELISA. C . Western blot analysis of
IκBα levels in heart tissue. GAPDH was used as a loading control.D. Nuclei were isolated from mouse heart tissue, and NF-κB in
the nucleus was detected by western blot. Lamin B and GAPDH were used as
controls. E-G. Real-time PCR was used to determine the mRNA
levels of Tnfa (E), Mcp1 (F), and Il-6 (G) in mouse
heart tissue (data were normalized to Actb ). (n=7; ***P <0.001 vs WT control group; ###P <0.001 vs DOX group).