Figure 5. LCZ696 treatment and TLR2 knockdown attenuated the doxorubicin-induced H9C2 cell inflammatory response in vitro.
A-B. H9C2 cells were transfected with control siRNA, TLR2 siRNA and LCZ696 (20 μmol/l) and then stimulated with DOX (1 μM) for 8 h. Western blot detected IκBa and GAPDH protein levels in whole cell lysates (A) and detected NF-κB, Lamin B and GAPDH protein levels in the nuclear extractions (B); C. Densitometric quantification of IκBa/GAPDH levels in the immunoblots presented in Figure 5A; D.Densitometric quantification of NF-κB/Lamin B levels in the immunoblots presented in Figure 5B; E-G. The mRNA levels of Tnfa(E), Mcp1 (F), and Il-6 (G) were measured by real-time PCR. (n=5; *** P <0.001 vs Ctrl group; ###P <0.001 vs DOX group).