Figure 6. LCZ696 treatment attenuated doxorubicin-induced cardiac injury by inhibiting TLR2-MyD88 complex formation.
A. Time course of the DOX-induced TLR2-MyD88 interaction in H9C2 cells. Cells were treated with 1 µM DOX for up to 1 h. Quantification is shown below. B. H9C2 cells were pretreated with LCZ696 (20 μmol/l) for 1 h and then stimulated with DOX (1 μM) for 30 min. The TLR2-MyD88 interaction was analyzed. Quantification is shown below. C. H9C2 cells were transfected with control siRNA (NC) or TLR4 siRNA (siTLR4) and then treated with DOX (1 μM) for 15 min. TLR2-MyD88 interactions were analyzed. Quantification is shown below.D. H9C2 cells were transfected with control siRNA (NC) or MD2 siRNA (siMD2) and then treated with DOX (1 μM) for 15 min. TLR2-MyD88 interactions were analyzed. Quantification is shown below. (n=5; ***P <0.001 vs Ctrl group; ### P <0.001 vs DOX group).