Figure 4. LCZ696 treatment and TLR2 knockdown attenuated
doxorubicin-induced H9C2 cell fibrosis in vitro.
A. H9C2 cells were transfected with control siRNA (NC) or TLR2
siRNA (siTLR2) and treated with LCZ696 (20 μmol/l) for 24 h. Western
blot detected TLR2 and GAPDH protein levels. B. Densitometric
quantification of TLR2/GAPDH levels in the immunoblots presented in
Figure 4A. C. H9C2 cells were transfected with control siRNA or
TLR2 siRNA and pretreated with LCZ696 (20 μmol/l) and then stimulated
with DOX (1 μM) for 24 h. Western blot analysis of the protein levels of
collagen I and TGF-β in whole cell lysates. D. Densitometric
quantification of collagen I/GAPDH and TGF-β/GAPDH levels in immunoblots
presented in Figure 4C. E-F. The mRNA levels of Col1a1(E) and Tgfb (F) were detected by real-time PCR. (n=5; ***P <0.001 vs Ctrl group; ### P <0.001
vs DOX group)