Figure 4. LCZ696 treatment and TLR2 knockdown attenuated doxorubicin-induced H9C2 cell fibrosis in vitro.
A. H9C2 cells were transfected with control siRNA (NC) or TLR2 siRNA (siTLR2) and treated with LCZ696 (20 μmol/l) for 24 h. Western blot detected TLR2 and GAPDH protein levels. B. Densitometric quantification of TLR2/GAPDH levels in the immunoblots presented in Figure 4A. C. H9C2 cells were transfected with control siRNA or TLR2 siRNA and pretreated with LCZ696 (20 μmol/l) and then stimulated with DOX (1 μM) for 24 h. Western blot analysis of the protein levels of collagen I and TGF-β in whole cell lysates. D. Densitometric quantification of collagen I/GAPDH and TGF-β/GAPDH levels in immunoblots presented in Figure 4C. E-F. The mRNA levels of Col1a1(E) and Tgfb (F) were detected by real-time PCR. (n=5; ***P <0.001 vs Ctrl group; ### P <0.001 vs DOX group)