Reagents
Antibodies for FXR (Cat#: ab129089), LXR (Cat#: ab176323), GAPDH
(Cat#: ab181602), PPARγ(Cat#: ab178860), p-NFκb/P65 (Cat#: ab76302),
NFκb/P65 (Cat#: ab16502), and Bsep (Cat#: ab155421) were obtained from
Abcam (Cvambridge, MA). Reagents of Tectorigenin (TEC) (Cat#:
HY-N0792), GW9662 (Cat#: HY-16578), GW1929 (Cat#: HY-15655),
BAY11-7085 (Cat#: HY-10257) were obtained from MedChemExpress (Monmouth
Junction, NJ), and Lipopolysaccharides (LPS) (Cat#: L2630) and
alpha-naphtylisocyanate (ANIT) were obtained from Sigma-Aldrich
(Shanghai, China). Mouse AST ELISA Kit (Aspartate Aminotransferase)
(Cat#: ab263882), ALT Assay Kit (Cat#: ab241035), Caspase-3 Activity
Assay Kit (Cat#: ab252897), AP Assay Kit (Cat#: ab267583), and Gamma
Glutamyl Transferase (γ-GT) Assay Kit (Cat#: ab241029) were obtained
from Abcam (Cambridge, MA). Mouse IL-1β ELISA KIT (Cat#: SEKM-0002) was
obtained from Solarbio & Technology Co, Ltd (Beijing, China).
Fluorescein (FITC) Tunel Cell Apoptosis Detection Kit (Cat#: G1501),
Sirius Red dyestuff (Cat#:G1018), and HE dyestuff (Cat#: G1005) were
obtained from servicebio (Wuhan, China). FXR shRNA(m) (Cat#:
sc-155894), LXRα shRNA(m) (Cat#: sc-38829), PPARγ shRNA(h) (Cat#:
sc-29455-V), and PPARγ shRNA(m) (Cat#: sc-29456) were obtained from
Santa Cruz Biotechnology (Santa Cruz, CA). All other reagents were
obtained from Sigma-Aldrich except where indicated.