Figure 5. TEC restored LPS-induced insults on bile transporters in hepatocytes through PPARγ. (A-D) Hepatocytes cultured with LPS and/or TEC for 24 h. A, B: western blot was used to detect the phosphorylation of NFκb-p65, n=5. B, C: Chromatin immunoprecipitation (ChIP) of hepatocytes with specific antibodies for NFκb-p65 subunits binding to the promotor of mouse FXR (B) or the promotor of mouse LXR (C) represented as semiquantitative data (left) and quantitative PCR data with specific primers (right). D: the mRNA levels of FXR, LXR, Bsep and ABCG5 were determined by qRT-PCR, n=5. (E and F) Hepatocytes were transfected with shPPARγ and/or TEC in the presence of LPS. E: phosphorylation and protein level of NFκb-p65 was measured by western blot and quantitative data are shown on the right, n=5. F: qRT-PCR was used to determine the mRNA expression of FXR, LXR, Bsep and ABCG5, n=5. *p<0.05. The data represent the mean ± SD.