Data and Statistical Analysis
All data and statistical analysis comply with the recommendations on
experimental design and analysis in pharmacology (Curtis et al., 2018).
Data are presented as mean + standard errors of the mean (SEM).
Statistical differences between treatments were determined by analysis
of variance (ANOVA) followed by Tukeys multiple comparison post-hoc
tests where appropriate. One-way ANOVA were used for three of more
unmatched groups. Two-way ANOVA were used to analyse data when response
was influenced by two independent factors of interest. All statistical
analyses were performed using GraphPad PrismTM for Microsoft Windows®
(Versions 8, Graphpad software®, USA) where p <0.05 was
accepted as significant for all cases.