Strains |
|
|
E. coli DH5ɑ |
Clone host strain |
Transgen |
E. coli K-12 MG1655 |
The source of Eco-xylAB genes |
Lab
stock |
X. campestris |
The source
of Xca-xylAB genes |
Lab stock |
C. glutamicum SO26 |
Deletion of argF, ncgl1221,
argR, putP, iolR, and mscCG2; attenuation of
odhA, proB, pta, cat, and ncgl2228;
and overexpression of lysE, gdh, gdh2,
cg3035, pfkA, pyk, glt, tkt, and
iolT1
|
Lab stock |
Bacillus subtilis |
The source of pentose transporter araE
gene |
Lab stock |
C. glutamicum pX |
C. glutamicum SO26 with pXMJ19 |
This
study |
C. glutamicum EAB |
C. glutamicum SO26 with
p19Plac-EcoAB |
This study |
C. glutamicum XAB |
C. glutamicum SO26 with
p19Plac-XcaAB |
This study |
C. glutamicum XAB01 |
XAB with the knocking of araE gene
into the locus of iolR
|
This study |
C. glutamicum XAB02 |
XAB01with PH36 promoter in
the upstream of pepc gene and 1957-1959 bp CGC replaces AAG in
pepc gene |
This study |
C. glutamicum XAB03 |
XAB02 with 895-897 bp AAC replaces GAT in
pepc gene |
This study |
Plasmids |
|
|
pK18mobsacB |
The suicide vector
containing the B. subtilis sacB gene;
kanR, allows for
selection of double crossover in C. glutamicum
|
Lab
stock |
pXMJ19 |
High copy expression vector,CmR,LacIq
promoter,tac promoter |
Lab stock |
p19Plac-EcoAB |
A derivative of pXMJ19, harboring
the
xylAB operon from E. coli MG1655 |
This
study |
p19Plac-XcaAB |
A derivative of pXMJ19, harboring
the
xylAB operon from X. campestris
|
This
study |
pk18-Peftu-△iolR::araE |
Using pK18mobsacB and araE to connect through the upper
and lower homology arms of △iolR, and add Peftu
promoter in the upstream of araE gene |
This study |
pk18-PH36-pepcTI |
A originated from pK18mobsacB,
add PH36 promoter in the upstream of pepc gene and
1957-1959 bp CGC replaces AAG in pepc gene |
This
study |
pk18-PH36-pepcT2 |
On the basis of
pk18-PH36-pepcTI, harboring
895-897 bp AAC replaces GAT in
pepc gene |
This study |