Strains/plasmids Relevant characteristics Source
Strains
E. coli DH5ɑ Clone host strain Transgen
E. coli K-12 MG1655 The source of Eco-xylAB genes Lab stock
X. campestris The source of Xca-xylAB genes Lab stock
C. glutamicum SO26 Deletion of argF, ncgl1221, argR, putP, iolR, and mscCG2; attenuation of odhA, proB, pta, cat, and ncgl2228; and overexpression of lysE, gdh, gdh2, cg3035, pfkA, pyk, glt, tkt, and iolT1 Lab stock
Bacillus subtilis The source of pentose transporter araE gene Lab stock
C. glutamicum pX C. glutamicum SO26 with pXMJ19 This study
C. glutamicum EAB C. glutamicum SO26 with p19Plac-EcoAB This study
C. glutamicum XAB C. glutamicum SO26 with p19Plac-XcaAB This study
C. glutamicum XAB01 XAB with the knocking of araE gene into the locus of iolR This study
C. glutamicum XAB02 XAB01with PH36 promoter in the upstream of pepc gene and 1957-1959 bp CGC replaces AAG in pepc gene This study
C. glutamicum XAB03 XAB02 with 895-897 bp AAC replaces GAT in pepc gene This study
Plasmids
pK18mobsacB The suicide vector containing the B. subtilis sacB gene; kanR, allows for selection of double crossover in C. glutamicum Lab stock
pXMJ19 High copy expression vector,CmR,LacIq promoter,tac promoter Lab stock
p19Plac-EcoAB A derivative of pXMJ19, harboring
the xylAB operon from E. coli MG1655 This study
p19Plac-XcaAB A derivative of pXMJ19, harboring
the xylAB operon from X. campestris This study
pk18-Peftu-△iolR::araE Using pK18mobsacB and araE to connect through the upper and lower homology arms of △iolR, and add Peftu promoter in the upstream of araE gene This study
pk18-PH36-pepcTI A originated from pK18mobsacB, add PH36 promoter in the upstream of pepc gene and 1957-1959 bp CGC replaces AAG in pepc gene This study
pk18-PH36-pepcT2 On the basis of pk18-PH36-pepcTI, harboring 895-897 bp AAC replaces GAT in pepc gene This study