Knocking-in the pentose transporter
demonstrates the promotion of xylose utilization and L-ornithine
production
In order to increase the rate of xylose consumption, the pentose
transporter gene (araE ) from Bacillus subtilis was
integrated into the genome locus ofiolR with the strong Peftu promoter, to generate
the strain termed as XAB01. The pentose transporter AraE is not only
extremely significant for the arabinose transportation, but also
promotes the transportation of xylose to cells (Mao et al., 2018). As
expected, the results showed that strain XAB01 demonstrated superior
xylose consumption and L-ornithine synthesis than strain XAB (Fig.3a and
3b). strain XAB01 consumed 47.9 g/L xylose after 72h fermentation, with
an average consumption rate of 0.665 g/(L·h). Through the rapid
utilization of xylose, the growth and L-ornithine yield of strain were
also improved. The output of L-ornithine increased by 12.5% (24.3 ±
0.23g/L) compared with strain XAB, and the xylose yield is 0.51 g/g.