4. Conclusion:
In this study, qHNMR was used to quantify epoxy methyl oleate prepared
from oleic acid. Two different 1H NMR equations have
been proposed for the quantification of EMO in EMO and MO mixture. The
EMO quantification values obtained from equation 2 was found to
demonstrate better correlation (0.999) with that of EMO present in the
standard mixture of EMO and MO. The results obtained from the1H NMR technique are also in accordance with that of
HPLC (R2 = 0.996). The developed equation 2has also been applied on the quantification of the real EMO sample
obtained from the epoxidation of methyl oleate. The linearity,
reproducibility and validity of the qHNMR technique suggest that qHNMR
could be used as a reliable and quick method for the quantification of
epoxy methyl oleate (EMO).