3.4.2 Reproducibility and Linearity:
A linearity curve for both the derived equations was also evaluated (Fig. 4 ) which showed the correlation coefficient of > 0.999. To test the reproducibility and linearity of the method, three different technical hands have followed the identical experimental procedure to quantify the EMO in the mixture of MO/EMO and employed equation 1 and 2 . The observed results showed good correlation with the true concentrations confirming the excellent reproducibility of the method (Table 1a, supporting information). As shown in Table 1 , as the EMO concentration in the mixture increases beyond 70%, both the equations were found to be consistent with the actual values. Nevertheless, equation 2 was found to be more consistent with actual concentration as well as with HPLC analysis at all concentration levels.