Figure (4): Immunoflorescence detection of occludin in
testicular tissue.
Photomicrograph of transverse section of rat showing immunoflorescence
detection of occludin in testicular tissue (X 20). (A)Immunofluorescent staining of occludin in rat testicular section from
control group showing normal nuclei (stars) stained blue (DAPI), and
normal interstitial expression and distribution of occludin (green
fluorescence; Cruz Fluor 488) (arrows). (B) Immunofluorescent
staining of occludin in rat testicular section from TQ-treated group
showing normal nuclei (stars) stained blue (DAPI), and normal
interstitial expression and distribution of occludin (green
fluorescence; Cruz Fluor 488) (arrows). (C) Immunofluorescent
staining of occludin in rat testicular section from capsaicin-treated
group showing normal nuclei (stars) stained blue (DAPI), and normal
interstitial expression and distribution of occludin (green
fluorescence; Cruz Fluor 488) (arrows). (D) Immunofluorescent
staining of occludin in rat testicular section from
acrylamide-challenged group showing abnormal expression and distribution
of occludin interstitially (arrows) (lower interstitial fluorescent
green color intenisty) and also abnormal nuclear (stars) distribution
(higher nuclear fluorescent green color intenisty). (E)Immunofluorescent staining of occludin in rat testicular section from
acrylamide-challenged group that concomitantly received TQ. The section
depicts restoration of the expression of occludin interstitially (as
marked by higher green fluorescence (arrows) than nuclear expression
(stars). (F) Immunofluorescent staining of occludin in rat
testicular tissue from acrylamide-challenged group that concurrently
received capsaicin. The section shows increased interstitial green
fluorescence intensity and distribution of occludin (arrows) higher than
nuclear distribution and expression of the junctional protein (stars).