Effects of treatment regimens on testicular catalase (CAT)
activity in acrylamide-challenged rats
Acrylamide, however, induced dramatic decrease in the enzymatic activity
amounted to 83% compared to control rats. Concurrent administration of
either TQ or capsaicin to the animals given acrylamide resulted in
notable increases in the enzymatic activities in testicular tissue
amounted to about 236% and 198%, respectively compared to the group
that received acrylamide alone.
Further, the group co-treated with capsaicin and acrylamide exhibited
lower but significant decrease in CAT activity by about 11% compared to
the animals that received TQ and acrylamide (Table 3) .
Effects of treatment regimens on histopathological examination
of the testicular tissue specimens
The results are presented as a photomicrograph in figure (2) and
severity of histopathological alterations in testicular tissue specimens
of different experimental groups was shown.
Effects of treatment regimens on immunohistochemistry
localization of nuclear factor (NF-κB /p65) in testicular tissueThe results are presented as a photomicrograph of testicular tissue
specimens in figure (3), and intensity of immuno-histological
reactions of NF-κB/p65 in of testicular tissue specimens of different
experimental groups was shown in table (4).
Acrylamide induced diffuse strong positive immunoreaction of NF-κB/p65
(brown color) in rat testicular tissue. There was dramatic
increase in reciprocal intensity amounted to 37-fold compared to normal
tissue. Tissue sections obtained from the group co-treated with TQ and
acrylamide demonstrated that most of the seminiferous tubules were
intact with complete spermatogenic series, and showed weak positive
immunoreaction for NF-κB/p65. Reciprocal intensity as a measure
of the degree of staining was significantly reduced by about 51%
compared to acrylamide-treated animals. Nevertheless, the reciprocal
activity was still higher than the normal testicular tissue by 18-fold
(Figure 3 and Table 4 ).
Likewise, concurrent administration of capsaicin and acrylamide
exhibited less immunoreactivitiy than animals challenged with acrylamide
alone. Besides, the reciprocal intensity was significantly reduced by
47% compared to that of acrylamide group. Further, there was no
significant difference in the expression profile of NF-κB/p65 following
the combination modalities that incorporated acrylamide with either TQ
or capsaicin (Figure 3 and Table 4 ).
Effects of treatment regimens on immunoflorescence detection
of occludin in testicular tissue
The results are presented as a photomicrograph of testicular tissue
specimens in figure (4), and fluorescence intensity of occludin in of
testicular tissue specimens of different experimental groups were shown
in table (4).
Following acrylamide challenge, there was a marked nadir in the
expression of the junctional protein as shown from the apparent decrease
in the green fluorescence intensity amounted to 55% compared to control
animals associated with loss of most of the integrity of basement
membrane surrounding the seminiferous tubules. Administration of TQ to
acrylamide-treated rats significantly increased the expression of
interstitial occludin by about 99% compared to acrylamide-challenged
animals, and almost restored it to the normal expression pattern
(Figure 4 and Table 4 ).
By the same token, concomitant administration of capsaicin with
acrylamide resulted in notable increase in the expression of occludin at
the basement membrane by about 110% compared to rats that received
acrylamide alone, and almost brought back the junctional protein
expression to the normal level (Figure 4 and Table 4 ).