Assessment of sperm count and motility
Assessment of sperm count and motility was achieved according to the routine of Freund and Carol(Freund & Carol, 1964). The two cauda epididymis from each rat were situated in 2 ml of warmed (37 C) Earle’s buffer. Sperm count and motility were inspected using hemocytometer and the light microscope as expressed by Hoppe and Pitts (Hoppe & Pitts, 1973).