Histopathological examination of the testes
Necropsy samples were taken from the testes of rats in the different
groups of the work and fixed in Bouin’s solution for twenty-four hours.
Washing was done using tap water then serial dilutions of alcohol
(methyl, ethyl and absolute ethyl) were used for dehydration. Specimens
were cleared in xylene and embedded in paraffin at 56 degree in hot air
oven for twenty-four hours. Paraffin bees wax tissue blocks were
prepared for sectioning at 4 microns by microtome. The obtained tissue
sections were collected on glass slides, deparffinized and stained by
hematoxylin and eosin stains (Banchrof,
Steven, & Turner, 1996) for histopathological examination through the
light microscope.