Determination of serum testosterone concentration
The DRG Testosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with an antibody directed towards a unique antigenic site on the testosterone molecule. Endogenous testosterone of the sample competes with the testosterone horseradish peroxidase conjugate for binding to the coated antibody. After incubation for 15 minutes, the unbound conjugate is washed off. The amount of bound testosterone horseradish peroxidase conjugate is reversely proportional to the concentration of testosterone in the sample. After addition of the substrate solution, the intensity of the developed color is reversely proportional to the concentration of testosterone in the given sample (Tietz, 1986).