Determination of thiobarbituric acid reactive substances (TBARS) contents calculated as malondialdehyde (MDA)
Colorimetric determination of TBARS [calculated as MDA content] is based on the reaction of one molecule of MDA with two molecules of thiobarbituric acid at low pH (2-3), and a temperature of 95ÂșC for 45 min. The resultant pink color was extracted with n-butanol, and the absorbance was determined at 535 nm and 520 nm spectrophotometrically (Uchiyama & Mihara, 1978).