Figure (4): Immunoflorescence detection of occludin in testicular tissue.
Photomicrograph of transverse section of rat showing immunoflorescence detection of occludin in testicular tissue (X 20). (A)Immunofluorescent staining of occludin in rat testicular section from control group showing normal nuclei (stars) stained blue (DAPI), and normal interstitial expression and distribution of occludin (green fluorescence; Cruz Fluor 488) (arrows). (B) Immunofluorescent staining of occludin in rat testicular section from TQ-treated group showing normal nuclei (stars) stained blue (DAPI), and normal interstitial expression and distribution of occludin (green fluorescence; Cruz Fluor 488) (arrows). (C) Immunofluorescent staining of occludin in rat testicular section from capsaicin-treated group showing normal nuclei (stars) stained blue (DAPI), and normal interstitial expression and distribution of occludin (green fluorescence; Cruz Fluor 488) (arrows). (D) Immunofluorescent staining of occludin in rat testicular section from acrylamide-challenged group showing abnormal expression and distribution of occludin interstitially (arrows) (lower interstitial fluorescent green color intenisty) and also abnormal nuclear (stars) distribution (higher nuclear fluorescent green color intenisty). (E)Immunofluorescent staining of occludin in rat testicular section from acrylamide-challenged group that concomitantly received TQ. The section depicts restoration of the expression of occludin interstitially (as marked by higher green fluorescence (arrows) than nuclear expression (stars). (F) Immunofluorescent staining of occludin in rat testicular tissue from acrylamide-challenged group that concurrently received capsaicin. The section shows increased interstitial green fluorescence intensity and distribution of occludin (arrows) higher than nuclear distribution and expression of the junctional protein (stars).