Determination of thiobarbituric acid reactive substances (TBARS)
contents calculated as malondialdehyde (MDA)
Colorimetric determination of TBARS [calculated as MDA content] is
based on the reaction of one molecule of MDA with two molecules of
thiobarbituric acid at low pH (2-3), and a temperature of 95ÂșC for 45
min. The resultant pink color was extracted with n-butanol, and the
absorbance was determined at 535 nm and 520 nm spectrophotometrically
(Uchiyama & Mihara, 1978).