Determination of serum testosterone concentration
The DRG Testosterone ELISA Kit is a solid phase enzyme-linked
immunosorbent assay (ELISA), based on the principle of competitive
binding. The microtiter wells are coated with an antibody directed
towards a unique antigenic site on the testosterone molecule. Endogenous
testosterone of the sample competes with the testosterone horseradish
peroxidase conjugate for binding to the coated antibody. After
incubation for 15 minutes, the unbound conjugate is washed off. The
amount of bound testosterone horseradish peroxidase conjugate is
reversely proportional to the concentration of testosterone in the
sample. After addition of the substrate solution, the intensity of the
developed color is reversely proportional to the concentration of
testosterone in the given sample (Tietz,
1986).