Tail vein injections
This animal study was conducted in accordance with the rules and
regulations of the IACUC at the Department of Laboratory Animal Science,
Fudan University
(Shanghai,
P.R. China).
Lewis
lung carcinoma (LLC)
cells
were collected and resuspended in serum-free medium. C57/BL6 mice were
injected
through the tail vein with
1×106cells in 0.1 mL. Fourteen days after injection of cells, the mice
were
subjected to PET scanning. Mice were euthanized by pentobarbital sodium,
and all lungs were dissected or further imaged by
fluorescence
stereomicroscopy and then were fixed in Bouin’s solution, and the number
of metastatic nodules on the lung surface was counted.
Subcutaneousinoculation
of tumors in mice
This animal study was conducted in accordance with the rules and
regulations of the IACUC at the Department of Laboratory Animal Science,
Fudan University (Shanghai, P.R. China).
Tumor
cells at a density of
1×106in 0.1mL serum-free media were injected subcutaneously into the right
flank of mice.
The
mice were monitored daily for the development of visible tumors. Once a
tumor was clearly visible, it was
calipered
three times each week, and the volume was estimated using the formula V
= (LW2)/2 where V is volume, L is longest diameter,
and W is shortest diameter.
Mice
were euthanized by pentobarbital sodium, and the tumors were removed,
some were fixed in 4% paraformaldehyde, and the other tumors were lysed
for Western blotting.