Figure Captions
Figure 1. (A) Relative adsorption of bovine serum albumin (BSA) on different adsorbent surfaces as (B) a function of the static water contact angle (θw ) and (C) the free energy of cohesion (ΔGiwi).
Figure 2. Idealized representation of the versatility of the Protein-Surface Interactions (adapted from (Contreras-Naranjo and Aguilar, 2019)).
Figure 3. (A) Freely suspended cell concentration of N. gaditana (g/ L) during the culture period for the different N/P ratios.(B) Phosphate concentration [PO4-] (μM) during the culture period for the different N/P ratios. (C) Nitrate concentration [NO3-] (mM) during the culture period for the different N/P ratios. Data points are averaged values, and vertical bars are the standard deviation for the two independent experiments and triplicate samples.
Figure 4. (A) Average cell adhesion (cells/mm2) between all surfaces, biomass concentration (g/L) and protein concentration (g/L) of the supernatant after 50 days. (B) the influence of the N/P ratio and type of material on cell adhesion (cells/mm2), obtained with a multifactorial ANOVA (C) Average cell adhesion (cells/mm) according to the position for each N/P ratio and multifactorial ANOVA result of position influence on the cell adhesion. The lowercase and capital letters in Figure 5B represent significant differences, with a p-value<0.05.
Figure 5. (A) Details of the vessel geometry and distribution of the coupons used for material testing. (B) Detail of the liquid-gas interface once the flow was developed. (C) Velocity vectors for a horizontal plane 5 µm above the coupons on the bottom and(D) Velocity vectors for a horizontal plane that passes through the middle of the coupons on the wall.
Figure 6. Relative cell adhesion (%) for each surface (NW, HX, H-X3, PS, PETG, PMMA and GS) and N/P ratio after 50 days, and the relative BSA adhesion (%) for each surface after 4 h.