Figure Captions
Figure 1. (A) Relative adsorption of bovine serum albumin (BSA)
on different adsorbent surfaces as (B) a function of the static
water contact angle (θw ) and (C) the
free energy of cohesion (ΔGiwi).
Figure 2. Idealized representation of the versatility of the
Protein-Surface Interactions (adapted from
(Contreras-Naranjo and Aguilar, 2019)).
Figure 3. (A) Freely suspended cell concentration of N.
gaditana (g/ L) during the culture period for the different N/P ratios.(B) Phosphate concentration
[PO4-] (μM) during the culture
period for the different N/P ratios. (C) Nitrate concentration
[NO3-] (mM) during the culture
period for the different N/P ratios. Data points are averaged values,
and vertical bars are the standard deviation for the two independent
experiments and triplicate samples.
Figure 4. (A) Average cell adhesion
(cells/mm2) between all surfaces, biomass
concentration (g/L) and protein concentration (g/L) of the supernatant
after 50 days. (B) the influence of the N/P ratio and type of
material on cell adhesion (cells/mm2), obtained with a
multifactorial ANOVA (C) Average cell adhesion (cells/mm)
according to the position for each N/P ratio and multifactorial ANOVA
result of position influence on the cell adhesion. The lowercase and
capital letters in Figure 5B represent significant differences, with a
p-value<0.05.
Figure 5. (A) Details of the vessel geometry and distribution
of the coupons used for material testing. (B) Detail of the
liquid-gas interface once the flow was developed. (C) Velocity
vectors for a horizontal plane 5 µm above the coupons on the bottom and(D) Velocity vectors for a horizontal plane that passes through
the middle of the coupons on the wall.
Figure 6. Relative cell adhesion (%) for each surface (NW, HX, H-X3,
PS, PETG, PMMA and GS) and N/P ratio after 50 days, and the relative BSA
adhesion (%) for each surface after 4 h.