2.6 Isolation of human and mouse spermatogenic cells
Spermatogenic cells were obtained through cell diameter/density at unit
gravity using the STA-PUT velocity sedimentation method. In brief, human
and mouse germ cells were extracted from the testicular biopsy tissues
of male adults and adult C57BL/6 male mice. Next, spermatogenic cells
were resuspended in 25 ml of 0.5% BSA solution and filtered through an
80 mm mesh to remove cell aggregates. After passage through a mesh
filter, the cells were resuspended in buffer containing 0.5% BSA and
loaded in an STA-PUT velocity sedimentation cell separator (ProScience)
for gradient separation. Several germ cell populations were collected
for subsequent analysis.