Statistical analysis
Statistical analysis was performed using Graph pad PRISM version 8. The
differences in LTE4 in single samples were done using the two tailed
Mann-Whitney U-test and Kruskal-Wallis test. The degree of association
between urinary LTE4 levels and viral loads, antibody levels and other
laboratory tests was analyzed using Spearman correlation coefficient
test. Changes in the LTE4 levels throughout the course of illness was
compared using the Holm-Sidak method. Corrections for multiple
comparisons were completed using the Holm-Sidak method and the
significance value was set at 0.05 (alpha). A receiver-operator
characteristic (ROC) curves showing the area under the curve (AUC) were
generated to determine the discriminatory performance of LTE4 in
predicting those who will develop DHF during early illness. The
non-parametric Kruskal-Wallis (KW) test was used to analyze LTE4 levels
in different age groups.