Determining primary and secondary dengue
Dengue antibody assays were performed in 77 patients, who had a duration
of illness of more than 5 days, using a commercial capture-IgM and IgG
Enzyme- Linked Immunosorbent Assay (ELISA) (Panbio, Australia). Results
were interpreted according to the manufacturer’s instructions. According
to the WHO 2011 criteria, patients with an IgM: IgG ratio of
>1.2 were classified as having a primary dengue infection,
while patients with IgM: IgG ratios <1.2 were categorized
under secondary dengue infection. Based on these criteria 26 (34%) had
a primary dengue infection and 51(66%) had a secondary dengue
infection.