Statistical analysis
Statistical analysis was performed using Graph pad PRISM version 8. The differences in LTE4 in single samples were done using the two tailed Mann-Whitney U-test and Kruskal-Wallis test. The degree of association between urinary LTE4 levels and viral loads, antibody levels and other laboratory tests was analyzed using Spearman correlation coefficient test. Changes in the LTE4 levels throughout the course of illness was compared using the Holm-Sidak method. Corrections for multiple comparisons were completed using the Holm-Sidak method and the significance value was set at 0.05 (alpha). A receiver-operator characteristic (ROC) curves showing the area under the curve (AUC) were generated to determine the discriminatory performance of LTE4 in predicting those who will develop DHF during early illness. The non-parametric Kruskal-Wallis (KW) test was used to analyze LTE4 levels in different age groups.