2.10 | Wounded monolayer migration assay
SKI SMCs were pretreated with PDTC (10 μM) or pioglitazone (10 μM) for
48 hr before migration assay (Tong et al., 2016). DMSO acted as solvent
control. 106 cells/well in 12-well plates were seeded
in 0.2% FBS DMEM overnight to reach confluence, then scratch wounds
were applied to SMC monolayer with a pipette tip. Immediately after
scratching, the cells were treated with 10% FBS DMEM to stimulate cell
migration. Photographs were taken at 0 hr and 6 hr at three fixed
locations along the scratch with a light microscope and analyzed using
NIH Image J software.