2.10 | Wounded monolayer migration assay
SKI SMCs were pretreated with PDTC (10 μM) or pioglitazone (10 μM) for 48 hr before migration assay (Tong et al., 2016). DMSO acted as solvent control. 106 cells/well in 12-well plates were seeded in 0.2% FBS DMEM overnight to reach confluence, then scratch wounds were applied to SMC monolayer with a pipette tip. Immediately after scratching, the cells were treated with 10% FBS DMEM to stimulate cell migration. Photographs were taken at 0 hr and 6 hr at three fixed locations along the scratch with a light microscope and analyzed using NIH Image J software.