IVIG treatment significantly reduced the production of anti-collagen antibodies in CIA mice
IVIG is known to consist neutralizing antibodies targeting the Fab portion of pathogenic autoantibodies, a phenomenon previously described as an anti-idiotypic activities. Therefore, we analyzed the effect of IVIG in amelioration of anti-collagen antibodies in the plasma of CIA mice ±treatment (Fig.4).
As illustrated in Fig.4, ELISA examination of antibodies against collagen in IVIG-CIA group showed OD of 1.15±0.28 at 405nm, as compared to 1.56±0.31 OD and 1.76 ±0.7 OD in PBS or untreated CIA mice, respectively, P <0.001.