IVIG treatment, regulated circulating cytokines levels in CIA mice
A direct effect of the cytokines on the destruction of the joint or another mechanism beside recruitment of inflammatory cells has been shown [1,2,37]. We evaluated the circulating inflammatory cytokines (IFNγ, IL-1β, IL-17, IL-6, TNFα) levels in the CIA mice plasma upon treatment with IVIG, vehicle or non-treatment, Fig,5A-E. The inflammatory cytokines level in IVIG-treated CIA mice were significantly lower in comparison to the control PBS or non-treated mice for all of the tested cytokines , P <0.001. For example: IL-6 levels in the plasma of CIA mice treated with IVIG were found to be 896±168pg/ml in comparison to 13,999±917 pg/ml and 13,481±869 pg/ml, for non-treated CIA mice and PBS treated CIA mice, respectively,P <0.001. The IL-17 levels in the plasma of IVIG treated CIA mice were 1,173±497 pg/ml, whereas in the non-treated or PBS treated CIA mice the levels were 10,318±1,521 pg/ml and 11,487±371 pg/ml respectively, P <0.001. The TNFα levels in the CIA mice treated with IVIG were 2,164.28±652 pg/ml compared to 11,099±4176 pg/ml and 13,969±3,044 pg/ml in the non-treated CIA mice or PBS treated CIA mice, respectively, P <0.001.