We considered a minimum of five animals per group. The parameters used to display the results were mean and standard deviation. First, Shapiro-Wilk test was used for normal distribution evaluation. Then, outliers were removed using Grubbs test. Finally, the minimum significance level with 95% confidence interval (p) was calculated using Student’s T-test when comparing two groups or one-way analysis of variance (ANOVA) with Tukey-Kramer post-test when comparing multiple groups (multiplicity adjusted p-value). All statistical tests were performed on GraphPad Prism^® 6.01 software by GraphPad Software, Inc. (La Jolla, CA, USA).