All procedures and number of animals for this work were approved by the university’s animal ethical committee under the permit number 781. This study follows the ARRIVE guidelines [16].

Adult (8 weeks old) female C57BL/6 (n=20) and Balb/c (n=20) mice were obtained from the local animal breeding facility (Núcleo de Criação de Animais de Laboratório - NAL – UFF) and kept in the experimental animal-room of the Immunobiology Department during the whole protocol. Animals were kept in polystyrene cages with steel covers in a conventional environment with acidified water, which is widely used in animal colonies to prevent the spread of bacterial diseases [17, 18], and commercial mouse chow ad libitum (temperature of 22°C, ~60% humidity and 12‐hours light/ 12‐hours dark cycle) and each strain was divided in four groups of 5 mice (n=5 per group).

Mice were submitted to a food allergy induction protocol described by Teixeira (2009). In short 100µg of protein extract was introduced twice subcutaneously with a three-week interval. To the primary inoculation, 1mg of alum (Al(OH)₃) was added.