Fig 6. Serum reactivity of Balb/c mice sensitized with peanut extracts tested on ELISA plates adsorbed with A) CPE-BB2βME; B) CPE-BB; C) CPE-SB and D) CPE-TB / HCl. N = 5. p <0.05 = *; p <0.01 = **; p <0.001 = ***; p <0.0001 = ****
Next, we performed an inter-lineage comparison using the sera derived from Balb/c and C57BL/6 mice that were sensitized and tested on ELISA plates coated with the homologous extract. No inter-strain differences were observed in CPE-BB2ßME, or CPE- TB/HCl reactivity. However, CPE-BB and CPE-SB (p<0.05) induced higher antibody titers in C57BL/6 mice than in Balb/c (Fig 7).