Fig 5. Titers of C57Bl/6 mice sensitized with CPE tested on ELISA plates adsorbed with A) CPE-BB2βME; B) CPE-BB; C) CPE-SB and D) CPE-TB / HCl. N = 5. p <0.05 = *; p <0.01 = **; p <0.001 = ***; p <0.0001 = ****

Sera from Balb/c mice sensitized with one of the four CPE and tested on CPE-BB2βME adsorbed plates (Fig 6A) showed significant differences in reactivity. Although sera from CPE-BB2ßME and CPE-BB sensitized mice presented similar mean antibody titers the intragroup variation was significantly different. The first showed a high, while the second a low, titer dispersion. Serum from CPE-BB2ßME and CPE-BB sensitized animals developed significantly higher antibody titers than CPE-SB and CPE-TB/HCl (p<0.001). On CPE-BB adsorbed plates (Fig 6B), serum from CPE-BB developed significantly higher antibody titers than CPE-BB2ßME (p<0.05). The sera from these same four CPE sensitized animals presented no intergroup differences in reactivity when tested on CPE-SB (Fig 6C) or CPE-TB/HCl (Fig 6D) coated plates.